Nice View of CleanEx Exp : GSE976_DOC

[General] [References] [Expression data]

General information about the entry
Dataset code GSE976
Dataset Title Gene Expression-Based High Throughput Screening: APL Treatment with Candidate Compounds
Description We developed a general approach to small molecule library screening called GE-HTS (Gene Expression-Based High Throughput Screening) in which a gene expression signature is used as a surrogate for cellular states and applied it to the identification of compounds inducing the differentiation of acute myeloid leukemia cells. In screening 1,739 compounds, we identified 8 that reliably induced the differentiation signature, and furthermore yielded functional evidence of bona fide differentiation. We tested several of these in duplicate replicates in blasts from a patient with APL. Also included in this data set are a collection of 6 primary patient AML cells, 3 normal neutrophils samples, and 3 normal monocyte samples. This data was used to evaluate whole genome effects of the compounds on APL cells in relation to AML versus normal neutrophils and monocytes..
Organism Homo sapiens
Format Type Intensity
Affymetrix;HG-U133A
Number of Experiments 24
Maximum log 9.76531378278884
Minimum log -6.30680512820116
References
Original URL http://www.ncbi.nlm.nih.gov/projects/geo/query/acc.cgi?acc=GSE976
Paper Reference Stegmaier K et al. Nat Genet. 2004 Mar;36(3):257-63. Epub 2004 Feb 8.
MEDLINE Reference 14770183
Experiments
Experiment numberExperiment CodeExperiment Description
1GSE976_GSM15272APL1;src:primary APL cells;agent=none;cell type=acute promyelocytic leukemia;";
2GSE976_GSM15394APL2;src:primary APL cells;agent=none;cell type=acute promyelocytic leukemia;";
3GSE976_GSM15395APL_RA1;src:primary APL cells treated with ATRA;agent=positive control;cell type=acute promyelocytic leukemia;";
4GSE976_GSM15412APL_RA2;src:primary APL cells treated with ATRA;agent=positive control;cell type=acute promyelocytic leukemia;";
5GSE976_GSM15413APL_Keto1;src:primary APL cells treated with 16-ketoestradiol;agent=16-ketoestradiol;cell type=acute promyelocytic leukemia;";
6GSE976_GSM15414APL_Keto2;src:primary APL cells treated with 16-ketoestradiol;agent=16-ketoestradiol;cell type=acute promyelocytic leukemia;";
7GSE976_GSM15415APL_Phen1;src:primary APL cells treated with 1,10-phenanthroline;agent=1,10-phenanthroline;cell type=acute promyelocytic leukemia;";
8GSE976_GSM15416APL_Phen2;src:primary APL cells treated with 1,10-phenanthroline;agent=1,10-phenanthroline;cell type=acute promyelocytic leukemia;";
9GSE976_GSM15417APL_EGFR1;src:prikmary APL cells treated with 4,5-dianilinophthalimide;agent=4,5-dianilinophthalimide;cell type=acute promyelocytic leukemia;";
10GSE976_GSM15418APL_EGFR2;src:primary APL cells treated with 4,5-dianilinophthalimide;agent=4,5-dianilinophthalimide;cell type=acute promyelocytic leukemia;";
11GSE976_GSM15419APL_Erythro1;src:primary APL cells treated with erythro-9-(2-hydroxy-3-nonyl) adenine HCl;agent=erythro-9-(2-hydroxy-3-nonyl)adenine HCl;cell type=acute promyelocytic leukemia;";
12GSE976_GSM15420APL_Erythro2;src:primary APL cells treated with erythro-9-(2-hydroxy-3-nonyl) adenine HCl;agent=erythro-9-(2-hydroxy-3-nonyl)adenine HCl;cell type=acute promyelocytic leukemia;";
13GSE976_GSM15421AML1;src:primary patient AML cells;agent=none;cell type=acute myelogenous leukemia;";
14GSE976_GSM15422AML2;src:primary patient AML cells;agent=none;cell type=acute myelogenous leukemia;";
15GSE976_GSM15423AML3;src:primary patient AML cells;agent=none;cell type=acute myelogenous leukemia;";
16GSE976_GSM15424AML4;src:primary patient AML cells;agent=none;cell type=acute myelogenous leukemia;";
17GSE976_GSM15425AML5;src:primary patient AML cells;agent=none;cell type=acute myelogenous leukemia;";
18GSE976_GSM15426AML6;src:primary patient AML cells;agent=none;cell type=acute myelogenous leukemia;";
19GSE976_GSM15427poly1;src:normal human neutrophils;agent=none;cell type=neutrophil;";
20GSE976_GSM15428poly2;src:normal human neutrophils;agent=none;cell type=neutrophil;";
21GSE976_GSM15429poly3;src:normal human neutrophils;agent=none;cell type=neutrophil;";
22GSE976_GSM15430mono1;src:normal human monocytes;agent=none;cell type=monocyte;";
23GSE976_GSM15431mono2;src:nomral human monocytes;agent=none;cell type=monocyte;";
24GSE976_GSM15432mono3;src:normal human monocytes;agent=none;cell type=monocyte;";