Nice View of CleanEx Exp : GSE6131_DOC

[General] [References] [Expression data]

General information about the entry
Dataset code GSE6131
Dataset Title Role of E2F3 expression in human bladder and prostate cancer cells
Description Amplification and overexpression of the E2F3 gene at 6p22 in human bladder cancer is associated with increased tumour stage, grade and proliferation index, and in prostate cancer E2F3 overexpression is linked to tumour aggressiveness. We first used small interfering RNA technology to confirm the potential importance of E2F3 overexpression in bladder cancer development. Knockdown of E2F3 expression in bladder cells containing the 6p22 amplicon strongly reduced the extent of bromodeoxyuridine (BrdU) incorporation and the rate of cellular proliferation. In contrast, knockdown of CDKAL1/ FLJ20342, another proposed oncogene, from this amplicon had no effect. Expression cDNA microarray analysis on bladder cancer cells following E2F3 knockdown was then used to identify genes regulated by E2F3, leading to the identification of known E2F3 targets such as Cyclin A and CDC2 and novel targets including pituitary tumour transforming gene 1, Polo-like kinase 1 (PLK1) and Caveolin-2. For both bladder and prostate cancer, we have proposed that E2F3 protein overexpression may cooperate with removal of the E2F inhibitor retinoblastoma tumor suppressor protein (pRB) to drive cellular proliferation. In support of this model, we found that ectopic expression of E2F3a enhanced the BrdU incorporation, a marker of cellular proliferation rate, of prostate cancer DU145 cells, which lack pRB, but had no effect on the proliferation rate of PC3 prostate cancer cells that express wild-type pRB. BrdU incorporation in PC3 cells could, however, be increased by overexpressing E2F3a in cells depleted of pRB. When taken together, these observations indicate that E2F3 levels have a critical role in modifying cellular proliferation rate in human bladder and prostate cancer..
Organism Homo sapiens
Format Type Dual_channel
Number of Experiments 3
Maximum log ratio 3.26935824359407
Minimum log ratio -2.72465027173297
Red channel log scale from 5.20945336562895 to 14.9537872221701
Green channel log scale from 5.20945336562895 to 14.4407394230143
References
Original URL http://www.ncbi.nlm.nih.gov/projects/geo/query/acc.cgi?acc=GSE6131
Paper Reference Olsson AY et al. Oncogene. 2007 Feb 15;26(7):1028-37. Epub 2006 Aug 14.
MEDLINE Reference 16909110
Experiments
Experiment numberExperiment CodeExperiment Description
1GSE6131_GSM14236032K 5637 E2F3 si3_0003;Slides were incubated at 651C for 1 h and then transferred to 42C for 72 h.;channel 2 : 5637_E2F3_si_scrambled(Bladder Cell line 5637 treated with a scrambled siRNA);channel 2 : gender: unknown;channel 2 : tissue type: tumor";
2GSE6131_GSM14235932K 5637 E2F3 si3_0002;Slides were incubated at 651C for 1 h and then transferred to 42C for 72 h.;channel 2 : 5637_E2F3_si_scrambled(Bladder Cell line 5637 treated with a scrambled siRNA);channel 2 : gender: unknown;channel 2 : tissue type: tumor";
3GSE6131_GSM14235832K 5637 E2F3 si3_0001 #2;Slides were incubated at 651C for 1 h and then transferred to 42C for 72 h.;channel 2 : 5637_E2F3_si_scrambled(Bladder Cell line 5637 treated with a scrambled siRNA);channel 2 : gender: unknown;channel 2 : tissue type: tumor";